DH5α (containing pCMV-ER-sfGFP-KDEL-Neo plasmid) Escherichia coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	37℃; 18-24 hours; Aerobic;
Storage Conditions	-80℃
Procedure	① Thaw the cryovial in a water bath (mold at 30℃, bacteria at 37℃) and shake rapidly to dissolve; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, then transfer it to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate all the dissolved liquid, and inoculate onto 2 corresponding antibiotic-resistant plates, approximately 200μL per plate; ④ Incubate the antibiotic-resistant plates under the specified conditions above for 18-24h, then observe (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate into 50mL of liquid LB antibiotic-resistant Recommended Medium, culture overnight at 37℃ on a shaker; ⑥ According to experimental needs, aspirate an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing