Stbl3 (containing pAAV-U6-sgRNA1-U6-sgRNA2-cTNT-Cre plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (molds at 30°C, bacteria at 37°C) and shake rapidly to dissolve; ② Disinfect the outer surface of the cryovial with an alcohol swab, then transfer it to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate the entire dissolved solution, and inoculate onto 2 plates with the corresponding antibiotic, approximately 200 μL per plate; ④ Incubate the antibiotic plates under the specified conditions above for 18-24h before observation (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate into 50 mL of liquid LB antibiotic Recommended Medium, culture overnight at 37°C on a shaking incubator; ⑥ According to experimental needs, take an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing

Ordering & shipping

• Minimum order: 1 unit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD; specification / size is listed on this page