Escherichia coli TOP10(pUC57-CA)
TOP10大肠杆菌含（pUC57-CA）
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TOP10 E. coli containing (pUC57-CA) - Basic Information
Recommended Medium Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃) Growth Conditions 37℃;
18-24h;
AerobicStorage Conditions -80℃ Procedure
① Thaw the cryovial in a water bath (mold 30℃, bacteria 37℃) with rapid shaking to dissolve;
② Wipe the outside of the cryovial with an alcohol swab for disinfection, then transfer to a biosafety cabinet for handling;
③ Unscrew the cap, aspirate all the dissolved liquid, and inoculate onto 1-2 agar plates (about 200μL per plate; for anaerobic bacteria, the Recommended Medium should be placed in an anaerobic environment for 24h in advance to remove oxygen);
④ Spread evenly and incubate under the above culture conditions.Sharing Method Public Welfare Sharing
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| Username | Quantity | bought time |
| Ya*** | 1 | 2026-09-07 |
| Um*** | 1 | 2026-08-28 |
| Ta*** | 1 | 2026-08-28 |
| Et*** | 1 | 2026-07-18 |
| Vi*** | 1 | 2026-07-06 |
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