DH5α (containing pCMV-MCS-P2A-mCherry-3×NLS(repeat-opt)-Neo plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (30°C for fungi, 37°C for bacteria) with rapid shaking to dissolve; ② Disinfect the outer wall of the cryovial with an alcohol swab, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate the entire dissolved solution, and spread onto 2 plates with the corresponding antibiotic, approximately 200 μL per plate; ④ Place the antibiotic plates under the above specified conditions and incubate for 18-24 h before observation (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate into 50 mL of liquid LB antibiotic Recommended Medium, culture overnight at 37°C on a shaker; ⑥ According to experimental needs, take an appropriate amount of the bacterial culture for plasmid extraction;
Sharing Method	Public welfare sharing