DH5α (Containing pZE21-MCS Plasmid) E. coli - Basic Information

Recommended Medium	Yeast Extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (mold at 30°C, bacteria at 37°C) with rapid shaking to dissolve; ② Wipe the outside of the cryovial with an alcohol swab for disinfection, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap and draw out all the dissolved liquid, then inoculate onto 1-2 agar plates (about 200μL per plate; for anaerobic bacteria, the Recommended Medium should be placed in an anaerobic environment for 24h in advance to remove oxygen); ④ Spread evenly and then incubate under the growth conditions described above.
Sharing Method	Public Welfare Sharing

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