DH5α (containing pSH47-HIS3 plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	37℃; 18-24h; Aerobic
Storage Conditions	-80℃
Procedure	① Thaw the cryovial in a water bath (molds at 30℃, bacteria at 37℃) and shake rapidly to dissolve; ② Wipe the outer wall of the cryovial with an alcohol cotton pad for disinfection, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, draw out all the dissolved liquid, and inoculate onto 1-2 agar plates (about 200μL per plate; for anaerobic bacteria, place the Recommended Medium in an anaerobic environment for 24h in advance to remove oxygen); ④ Spread evenly and incubate under the above culture conditions.
Sharing Method	Public Welfare Sharing

Ordering & shipping

• Minimum order: 1 unit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD; specification / size is listed on this page