DH5α (containing pCold I plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (mold: 30°C, bacteria: 37°C) and shake rapidly to dissolve; ② Wipe the outside of the cryovial with an alcohol pad for disinfection, then transfer it to a biosafety cabinet for further handling; ③ Unscrew the cap, pipette out the entire dissolved suspension, and spread it onto two plates with the appropriate antibiotic, about 200 μL per plate; ④ Incubate the antibiotic plates under the specified conditions for 18-24 h and then observe (do not seal or wrap the plates); ⑤ Pick single colonies and inoculate them into 50 mL liquid LB with the appropriate antibiotic as the Recommended Medium, and culture at 37°C on a shaker overnight; ⑥ According to experimental needs, take an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public welfare sharing

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