Stbl3 (containing pSIN-EF1a-MCS-P2A-EGFP-Neo plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (mold: 30°C, bacteria: 37°C), shaking rapidly to dissolve; ② Disinfect the outer wall of the cryovial with an alcohol swab, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate all the dissolved liquid, and spread onto 2 corresponding antibiotic-resistant plates, approximately 200 μL per plate; ④ Incubate the antibiotic-resistant plates under the specified conditions above for 18-24h, then observe (do not seal or wrap the plates); ⑤ Pick single colonies and inoculate into 50 mL liquid LB antibiotic-containing Recommended Medium, culture overnight at 37°C on a shaker; ⑥ Based on experimental needs, aspirate an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing

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