DH5α (containing pT3-EF1a-HA plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (molds at 30°C, bacteria at 37°C) and shake rapidly to dissolve; ② Wipe the outside of the cryovial with an alcohol swab for disinfection, then transfer it to a biosafety cabinet for handling; ③ Unscrew the vial cap, withdraw all the dissolved solution, and inoculate it onto 2 plates with the corresponding antibiotic, approximately 200 µL per plate; ④ Incubate the antibiotic plates under the specified conditions above for 18-24 h before observation (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate it into 50 mL of liquid LB antibiotic Recommended Medium, then culture overnight at 37°C on a shaking incubator; ⑥ According to experimental needs, take an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing

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