DH5α (containing pCMV-N-Flag-BiolD2 plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	37℃; 18-24h; Aerobic
Storage Conditions	-80℃
Procedure	① Prepare 1 tube of sterile water and 2 pieces of 90mm antibiotic-resistant plates; ② Disinfect the surface of the ampoule tube and open it in a biosafety cabinet. Heat the top with an alcohol lamp, then quickly drop sterile water onto it to cause cracking, and subsequently break it with forceps; ③ Pipette 0.5mL of sterile water into the freeze-dried tube, fully dissolve it, then spread the suspension onto antibiotic-resistant plates, 200μL per plate; ④ Incubate the antibiotic-resistant plates under the specified conditions above for 18-24h, then observe (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate it into 50mL of liquid LB antibiotic Recommended Medium, culture overnight at 37℃ on a shaker; ⑥ According to experimental needs, take an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing

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