pscAAV8-GFP - Basic Information

Recommended Medium	Yeast Extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	37℃; 18-24 hours; Aerobic;
Storage Conditions	2-8℃
Procedure	① Dissolution: Add 20μL sterile water to the plasmid lyophilized powder to dissolve, and let stand at room temperature for 1 min; ② Mixing: Mix 200μL competent cells with 5-10μL plasmid DNA, and place on ice for 30 min; ③ Heat-shock transformation: Incubate at 42℃ for 90 s; ④ Membrane pore contraction: Place on ice for 2 min; ⑤ Recovery culture: Add 800μL liquid Recommended Medium to each tube, and culture at 37℃ on a shaking incubator for 1 h (150 r/min); ⑥ Selection culture: Spread an appropriate volume (100 μL) of recovered cells onto LB plates with the corresponding antibiotic, leave the plates upright for 30 min (ensure the agar surface is thoroughly dry), then invert and culture for 12-16 h until colonies appear; ⑦ Extraction: Pick single colonies into LB liquid Recommended Medium with the corresponding antibiotic, culture with shaking for 12-16 h, and extract the plasmid according to experimental needs.
Intended Use	pscAAV8-GFP is an adeno-associated virus serotype 8 (AAV8) backbone plasmid carrying the GFP (green fluorescent protein) reporter gene, used for efficient transduction of various tissues (such as liver and muscle) and for tracking viral transfection efficiency and gene expression through fluorescence.
Sharing Method	Public welfare sharing

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