pUCP18-EYFP-C1 - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 h; Aerobic
Storage Conditions	2-8°C
Procedure	① Dissolution: Add 20 μL of sterile water to the plasmid dry powder to dissolve it, and let it stand at room temperature for 1 min; ② Mixing: Take 200 μL of competent cells and mix with 5-10 μL of plasmid DNA, and place on ice for 30 min; ③ Heat-shock introduction: Place at 42°C for 90 s; ④ Membrane pore contraction: Place on ice for 2 min; ⑤ Recovery culture: Add 800 μL of liquid Recommended Medium to each tube, and culture at 37°C on a shaker for 1 h (150 r/min); ⑥ Screening culture: Spread an appropriate volume (100 μL) of recovered cells onto LB plates with the corresponding antibiotic resistance. Leave the plates upright for 30 min (the agar surface must be dry first), then invert and culture for 12-16 h until colonies appear; ⑦ Extraction: Pick a single colony into liquid Recommended Medium with the corresponding antibiotic resistance, culture with shaking for 12-16 h, and extract the plasmid as required for the experiment.
Reference Use	pUCP18-EYFP-C1 is a fluorescent protein expression plasmid suitable for broad-host-range use (such as Escherichia coli and Pseudomonas aeruginosa), with pUCP18 as its backbone vector. It achieves yellow fluorescent labeling by expressing EYFP (enhanced yellow fluorescent protein) and carries ampicillin resistance for selection.
Sharing Method	Public welfare sharing