pcDNA3 Flag-humanTRAF2 (1087) - Basic Information

Recommended Medium	Yeast Extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	37℃; 18 hours; Aerobic;
Storage Conditions	2-8℃
Procedure	1. Dissolution: After receiving the plasmid dried powder, add 20μl sterile water to the bottom of the tube to dissolve the plasmid, and let stand at room temperature for 1min; 2. Mixing (plasmid adsorption): Mix 200μl competent cells with 5~10µl plasmid DNA, and place on ice for 30min; 3. Heat shock introduction: Let stand at 42℃ for 90s; 4. Membrane pore contraction: Ice bath for 2min; 5. Recovery culture: Add 800µl LB liquid Recommended Medium to each tube, culture at 37℃ for 1h at 150 r/min; 6. Selection culture: Spread an appropriate volume (100 µl) of recovered cells onto LB plates with corresponding antibiotics, leave plates upright for 30min (agar surface must be dry), then invert and culture for 12-16h until colonies appear. 7. Extraction: Pick single monoclonal colonies into LB liquid Recommended Medium with corresponding antibiotics, culture with shaking for 12-16h, and extract plasmid as needed for experiments.
Reference Use	Expression of human TRAF2 gene in mammalian cells
Sharing Method	Public welfare sharing