pET32a-RbcL(Laminaria japonica Aresch)-Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	LB + Ampicillin, 37℃ (Cloning strain DH5α, 1464bp)
Storage Conditions	2-8℃
Procedure	1. Dissolution: After receiving the plasmid dry powder, add 20μl sterile water to the bottom of the tube to dissolve the plasmid, and let stand at room temperature for 1min; 2. Mixing (plasmid adsorption): Mix 200μl competent cells with 5~10µl plasmid DNA, and place on ice for 30min; 3. Heat-shock introduction: Let stand at 42℃ for 90s; 4. Membrane pore contraction: Ice bath for 2min; 5. Recovery culture: Add 800µl LB liquid Recommended Medium to each tube, culture at 37℃ for 1h at 150 r/min; 6. Selection culture: Spread an appropriate volume (100µl) of recovered cells onto LB plates with the corresponding antibiotic, leave plates upright for 30min (agar surface must be dry), then invert and culture for 12-16h until colonies appear. 7. Extraction: Pick single monoclonal colonies into LB liquid Recommended Medium with the corresponding antibiotic, culture with shaking for 12-16h, and extract the plasmid as needed for the experiment.
Reference Use	Vector host: E. coli, vector purpose: protein expression, gene species: unknown, gene type: ORF, prokaryotic resistance: Amp, fluorescent protein: unknown.
Sharing Method	Public welfare sharing

Ordering & shipping

• Minimum order: 1 unit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD; specification / size is listed on this page