pUC57-pNZ8148 Plasmid - Basic Information

Recommended Medium	Yeast Extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	LB + Antibiotics, 37℃
Storage Conditions	2-8℃
Procedure	① Dissolution: Add 20μL of sterile water to the plasmid dry powder to dissolve it, and let it stand at room temperature for 1 min; ② Mixing: Mix 200μL of competent cells with 5-10μL of plasmid DNA, and place on ice for 30 min; ③ Heat shock introduction: Let stand at 42℃ for 90 s; ④ Membrane pore contraction: Ice bath for 2 min; ⑤ Recovery culture: Add 800μL of liquid Recommended Medium to each tube, and culture on a shaker at 37℃ for 1 h (150 r/min); ⑥ Screening culture: Spread an appropriate volume (100 μL) of recovered cells onto LB plates with the corresponding antibiotic, let the plates stand upright for 30 min (make sure the agar surface is completely dry), then invert and culture for 12-16 h until colonies appear; ⑦ Extraction: Pick single monoclonal colonies into liquid Recommended Medium with the corresponding antibiotic, culture with shaking for 12-16 h, and extract the plasmid as required for the experiment.
Reference Use	For construction of fluorescent strains of Lactobacillus plantarum
Sharing Method	Public welfare sharing