pLV2-CMV-MCS2-3×Myc-Puro - Basic Information

Recommended Medium	Yeast Extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	37℃; 18-24 hours; Aerobic;
Storage Conditions	2-8℃
Procedure	① Dissolution: Add 20μL sterile water to the plasmid dry powder to dissolve, and let stand at room temperature for 1 min; ② Mixing: Mix 200μl competent cells with 5-10μl plasmid DNA and place on ice for 30 min; ③ Heat-shock transformation: Let stand at 42℃ for 90s; ④ Membrane pore contraction: Ice bath for 2 min; ⑤ Recovery culture: Add 800μl liquid Recommended Medium to each tube, and culture on a shaker at 37℃ for 1h (150 r/min); ⑥ Selection culture: Spread an appropriate volume (100 μl) of recovered cells onto LB plates with the corresponding antibiotic, leave the plates upright for 30 min (the agar surface must be dry first), then invert and culture for 12-16h until colonies appear; ⑦ Extraction: Pick single colonies into LB liquid Recommended Medium with the corresponding antibiotic, culture with shaking for 12-16h, and extract plasmids according to experimental needs.
Intended Use	pLV2-CMV-MCS2-3×Myc-Puro is a lentiviral expression vector that uses the CMV promoter to drive expression of the target gene in the multiple cloning site (MCS), fused with a 3×Myc tag at the N-terminus or C-terminus, and enables stable cell line selection through puromycin resistance.
Sharing Method	Public Welfare Sharing