pCMV-EIF3H (human) - EGFP - Neo - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	37℃; 18-24h; Aerobic
Storage Conditions	2-8℃
Procedure	① Dissolution: Add 20μL sterile water to the plasmid dry powder to dissolve, and let stand at room temperature for 1min; ② Mixing: Mix 200μl competent cells with 5-10μl plasmid DNA, and place on ice for 30min; ③ Heat shock introduction: Let stand at 42℃ for 90s; ④ Membrane pore contraction: Ice bath for 2min; ⑤ Recovery culture: Add 800μl liquid Recommended Medium to each tube, and culture on a shaker at 37℃ for 1h (150 r/min); ⑥ Screening culture: Spread an appropriate volume (100 μl) of recovered cells on an LB plate with the corresponding antibiotic, leave the plate upright for 30min (the agar surface must be dry), then invert and culture for 12-16h until colonies appear; ⑦ Extraction: Pick a single monoclonal colony into LB liquid Recommended Medium with the corresponding antibiotic, culture with shaking for 12-16h, and extract the plasmid according to experimental needs.
Reference Use	pCMV-EIF3H (human) - EGFP - Neo is a plasmid used for expressing human EIF3H protein tagged with green fluorescent protein (EGFP) in mammalian cells. It also carries a Neo resistance marker, facilitating the screening of stably transfected cell lines with G418.
Sharing Method	Public welfare sharing