pLV3-CMV-Flag-MCS - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25°C)
Growth Conditions	37°C； 18-24 hours； Aerobic；
Storage Conditions	2-8°C
Procedure	① Dissolution: Add 20μL sterile water to the plasmid dry powder for dissolution, let stand at room temperature for 1min； ② Mixing: Take 200μl competent cells and mix with 5-10μl plasmid DNA, place on ice for 30min； ③ Heat shock introduction: Let stand at 42°C for 90s； ④ Membrane pore contraction: Ice bath for 2min； ⑤ Recovery culture: Add 800μl liquid Recommended Medium to each tube, culture on a shaker at 37°C for 1h (150 r/min)； ⑥ Selection culture: Spread an appropriate volume (100 μl) of recovered cells onto LB plates with the corresponding antibiotic. Leave plates upright for 30min (the agar surface must be dry), then invert and culture for 12-16h until colonies appear； ⑦ Extraction: Pick single monoclonal colonies into liquid Recommended Medium (LB) with the corresponding antibiotic, culture with shaking for 12-16h, and extract the plasmid as needed for the experiment
Intended Use	pLV3-CMV-Flag-MCS is a lentiviral expression vector that uses the CMV promoter to drive expression of a target gene in the multiple cloning site (MCS) with an N-terminal Flag tag fusion, facilitating protein detection and immunoprecipitation experiments.
Sharing Method	Public Welfare Sharing