Candida albicans Genomic DNA - Basic Information

Culture Medium	Yeast extract powder: 3.0g, Malt extract: 3.0g, Glucose: 10.0g, Casein peptone: 5.0g, Agar: 20.0g, pH: 6.2±0.2 (25℃)
Growth Conditions	Culture temperature 30℃; Culture time 24-48 hours; Gas environment aerobic;
Storage Conditions	2-8℃
Morphology	Colony diameter 1-2mm, round, regular edges, opaque, milky white front, raised center, smooth surface, bright surface, viscous texture, moist texture, G+ (blue-purple), cocci, purity: pure
Isolation Source	Nail infection
Procedure	Before use, add anhydrous ethanol to Buffer GD and Wash Solution PW, refer to the bottle label for volume; 1. Take yeast cells (up to 5×10⁷ cells), centrifuge at 12,000rpm (13,400×g) for 1min, remove supernatant as much as possible (if the bacterial suspension is large, collect cell pellet into one centrifuge tube through multiple centrifugations); 2. Yeast cell wall disruption: Enzymatic method: Add 600μL Sorbitol buffer to the pellet, add approximately 50U Lyticase (self-provided, catalog no.: RT410), mix thoroughly. Treat at 30℃ for 30min. Centrifuge at 4,000rpm (1,500×g) for 10min, discard supernatant, collect pellet. Note: The above is the Lyticase dosage for 5×10⁷ yeast cells; adjust Lyticase concentration and incubation time according to yeast strain and cell count; 3. Resuspend the pellet in 200μL Buffer GA, mix thoroughly. If RNA removal is needed, add 4μL RNase A (100mg/mL) solution (self-provided, catalog no.: RT405-12), vortex for 15sec, let stand at room temperature for 5min; 4. Add 20μL Proteinase K solution, mix; 5. Add 220μL Buffer GB, mix by inverting thoroughly, place at 70℃ for 10min, solution should become clear, briefly centrifuge to remove droplets from tube cap. Note: A white precipitate may form when adding Buffer GB, which usually disappears at 70℃ and does not affect subsequent experiments. If the solution does not become clear, cell lysis may be incomplete, leading to low DNA yield and impure DNA; 6. Add 220μL anhydrous ethanol, mix by inverting thoroughly, a flocculent precipitate may appear, briefly centrifuge to remove droplets from tube cap; 7. Transfer the solution and flocculent precipitate from the previous step into an adsorption column CB3 (placed in a collection tube), centrifuge at 12,000rpm (13,400×g) for 30sec, discard waste liquid, place adsorption column CB3 back into the collection tube; 8. Add 500μL Buffer GD (check if anhydrous ethanol has been added before use) to adsorption column CB3, centrifuge at 12,000rpm (13,400×g) for 30sec, discard waste liquid, place adsorption column CB3 back into the collection tube; 9. Add 600μL Wash Solution PW (check if anhydrous ethanol has been added before use) to adsorption column CB3, centrifuge at 12,000rpm (13,400×g) for 30sec, discard waste liquid, place adsorption column CB3 back into the collection tube; 10. Repeat step 9; 11. Place adsorption column CB3 back into the collection tube, centrifuge at 12,000rpm (13,400×g) for 2min, discard waste liquid. Leave adsorption column CB3 at room temperature for several minutes to completely dry residual wash solution from the adsorption material. Note: This step aims to remove residual wash solution from the adsorption column; ethanol residue in the wash solution can affect subsequent enzymatic reactions (digestion, PCR, etc.); 12. Transfer adsorption column CB3 to a clean centrifuge tube, add 50-200μL Elution Buffer TE dropwise to the center of the adsorption membrane, let stand at room temperature for 2-5min, centrifuge at 12,000rpm (13,400×g) for 2min, collect solution into the centrifuge tube. Note: To increase genomic DNA yield, the collected solution can be reapplied to the adsorption column, let stand at room temperature for 2min, centrifuge at 12,000rpm (13,400×g) for 2min. Elution buffer volume should not be less than 50μL; too small a volume affects recovery efficiency. The pH of the elution buffer greatly influences elution efficiency. If using ddH₂O as eluent, ensure its pH is within 7.0-8.5; pH below 7.0 reduces elution efficiency; DNA products should be stored at -20℃ to prevent degradation;
Sharing Method	Public welfare sharing

📋 Notes:

1. Storage Conditions:Lyophilized powder should be stored at 2–8°C away from light. After reconstitution or for solution-type products, store at –20°C and avoid repeated freeze-thaw cycles. For short-term frequent use (within 1 week), temporary storage at 4°C is acceptable.
2. Reconstitution Method:Prior to use, briefly centrifuge the lyophilized powder (e.g., 12,000 rpm for 1 minute) to collect the DNA at the bottom of the tube. It is recommended to dissolve in sterile TE buffer (pH 8.0) or sterile ddH₂O (pH 7.0–8.5). Mix gently; avoid vigorous vortexing.
3. Contamination Prevention:Gloves must be worn during operation. Use sterile, nuclease-free consumables. It is recommended to aliquot the reconstituted DNA into single-use portions before freezing, to prevent degradation caused by repeated freeze-thaw cycles and to avoid nuclease contamination.
4. Experimental Suitability:This product is suitable for common molecular biology applications such as PCR, qPCR, restriction enzyme digestion, and library construction. If the downstream assay is sensitive to inhibitors, ensure that the DNA solution is free of residual ethanol or high-concentration salt interference.
5. Intended Use Statement:The product is for research use only and is not intended for clinical diagnostic purposes.

Ordering & shipping

• Minimum order: 1 unit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD; specification / size is listed on this page