NE-4C Cell-Specific Culture Medium - Basic Information

Culture Medium	87% EMEM + 10% FBS + 1% Glutamine + 1% Sodium Pyruvate + 1% NEAA
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear, transparent, light red
Procedure	① Preparation: Expose pipettes, 50 mL centrifuge tubes, 500 mL serum bottles, and the clean bench to UV light for 20 minutes; pre-warm the basal medium in a 37°C constant temperature water bath. ② Mixing: Flame-sterilize the mouths of the basal medium bottle and serum bottle, then aseptically add 10% FBS + 1% Glutamine + 1% Sodium Pyruvate + 1% NEAA into the 87% basal medium. ③ Sealing: Seal the culture medium bottle mouth with sealing film, attach the corresponding product label, and place the culture medium back in a 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and expose to UV light for 30 minutes.

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