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Primary Mesenchymal Cell Culture System - Basic Information
Culture Medium Primary Mesenchymal Cell Culture System: 88% Primary Mesenchymal Cell Basal Medium + 1% Primary Mesenchymal Cell Culture Supplement + 10% FBS + 1% P/S Storage Conditions Store at 2-8°C, valid for one month Appearance Clear and transparent, light red Procedure ① Preparation: UV-irradiate the pipette, 50 mL centrifuge tube, 100 mL serum bottle, and clean bench for 20 minutes; pre-warm the basal medium in a 37°C water bath.
② Preparation: Flame-sterilize the mouths of the basal medium bottle and serum bottle, then aseptically pour the 1% Primary Mesenchymal Cell Culture Supplement, 10% FBS, and 1% P/S into 450 mL of basal medium.
③ Sealing: Seal the culture medium bottle with sealing film, attach the corresponding product label, and return the culture medium to a 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV-irradiate for 30 minutes.
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