scc-4 Cell-Specific Culture Medium - Basic Information

Culture Medium	90% DMEM-H/F12 + 10% FBS + 400ng/ml Hydrocortisone
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent
Procedure	① Preparation: Expose the pipette gun, 50mL centrifuge tube, 100mL serum bottle, and clean bench to UV light for 20 minutes; pre-warm the basal medium in a 37°C constant temperature water bath. ② Mixing: Flame-sterilize the openings of the basal medium bottle and serum bottle, then pour 10% FBS and 400ng/ml hydrocortisone into 90mL of basal medium while avoiding contamination. ③ Sealing: Seal the culture medium bottle opening with sealing film, attach the corresponding product label, and return the culture medium to the 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and irradiate with UV light for 30 minutes.