JIMT-1 Cell-Specific Culture Medium - Basic Information

Culture Medium	89% DMEM-H + 10% FBS + 1% NEAA
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear, transparent, pale red
Procedure	① Preparation: Sterilize the pipette, 50mL centrifuge tube, 100mL serum bottle, and the clean bench under UV light for 20 minutes; pre-warm the basal medium in a 37°C constant-temperature water bath. ② Preparation: Flame-sterilize the mouth of the basal medium bottle and the serum bottle, then aseptically add 50mL of serum and 1% NEAA into 450mL of basal medium. ③ Sealing: Seal the culture medium bottle with sealing film, attach the corresponding product label, and store the medium back in the 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and expose to UV light for 30 minutes.