L-15 Complete Medium (with 20% FBS) - Basic Information

Medium	80% L-15 + 20% FBS
Storage Conditions	Store at 2-8°C, valid for one year
Appearance	Clear, transparent, red
Procedure	① Preparation: Expose pipettes, 50 mL centrifuge tubes, 100 mL serum bottles, and the clean bench to UV light for 20 minutes; pre-warm the basal medium in a 37°C water bath. ② Preparation: Flame-sterilize the mouths of the basal medium bottle and serum bottle, then pour 100 mL of serum into 400 mL of basal medium without touching the rims. ③ Sealing: Seal the culture medium bottle with sealing film, attach the corresponding product label, and return the medium to the 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and expose to UV light for 30 minutes.