H-4-II-E Cell Dedicated Medium - Basic Information

Medium	89% EMEM + 10% FBS + 1% NEAA
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent
Procedure	① Preparation: Expose pipettes, 50mL centrifuge tubes, 100mL serum bottles, and the clean bench to UV light for 20 minutes; pre-warm the basal medium in a 37°C constant-temperature water bath. ② Formulation: Flame-sterilize the mouths of the basal medium bottle and serum bottle, then aseptically pour 50mL of serum and 5mL of NEAA into 445mL of basal medium. ③ Sealing: Seal the culture medium bottle mouth with parafilm, attach the corresponding product label, and return the culture medium to a 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and expose to UV light for 30 minutes.