IMDM Complete Medium (15% FBS) - Basic Information

Medium	85% IMDM + 15% FBS
Storage Conditions	2-8°C, protected from light
Appearance	Clear, transparent, red
Procedure	① Preparation: Sterilize pipettes, 50 mL centrifuge tubes, 500 mL serum bottles, and the clean bench under UV light for 20 minutes; pre-warm the basal medium in a 37°C constant-temperature water bath; ② Mixing: Flame-sterilize the openings of the basal medium bottle and serum bottle, then aseptically pour 15% FBS into 85% basal medium; ③ Sealing: Seal the culture medium bottle with parafilm, affix the corresponding product label, and store the medium back in the 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV-irradiate for 30 minutes.