Product Name: Mycobacterium bovis Karlson and Lessel

Recommended Medium	Middlebrook 7H9 Broth(Cat No.:SLPY16542)
Culture medium Composition	(NH4)2SO4:0.5g, KH2PO4:1.0g, Na2HPO4:2.5g, Na3C6H5O7:0.1g, MgSO4:0.05g, CaCl2:0.0005g, ZnSO4:0.001g, CuSO4:0.001g, L-Glutamic acid(C5H9NO4):0.5g, C6H8FeNO7:0.04g, Vitamin B6(C8H11NO3.HCl):0.001g, Biotin(C10H16N2O3S):0.0005g, ADC enrichment:10%(added at 50℃), pH:6.6±0.2(25℃)
Growth conditions	37 ° C; 3-4 weeks; aerobic
Storage conditions	2-8 ℃
Safety level	2
Colony Morphology	The colony diameter is 1-2mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, rough and wrinkled on the surface, dry texture, G+(blue purple), Bacillus, purity: pure

Product Form	Storage Condition	Shelf Life
Freeze-dried Powder	2~8℃	6 years
Agar Slant	2~8℃	1~3 months
Plate	4℃	7 days
Broth Culture	4℃	7 days
Cryopreserved Tube	-80℃	1~2 years

The shelf life stated above applies to unopened products stored under the recommended conditions. Actual shelf life may vary depending on the specific strain characteristics. Please contact our technical support for further details.

 

? Notes:

1. Product form: Freeze-dried powder is provided by default. For other forms (Agar Slant, Plate, or Broth Culture), please contact us for evaluation and quotation.
2. Inspection upon receipt: Please inspect the product immediately upon arrival. If any abnormality is found, contact our customer service within 24 hours of receipt. Failure to do so will be deemed as acceptance of the product in good condition.
3. Usage: The freeze-dried powder is for single use only and must not be stored after opening. After revival, subculture for 1–2 generations to restore full vitality before use.
4. Storage & shelf life: Please refer to the table above for recommended storage conditions and shelf life of each product form.
5. Disclaimer: Failure to follow the instructions for revival, subculture, and storage may result in abnormal growth or loss of viability. In such cases, no replacement will be provided.