SDS molecules have both hydrophilic and hydrophobic characteristics, and have important application value in the extraction, purification, and antigen preparation of genetically engineered protein drugs. However, SDS residues can have toxic side effects on animals and humans, so monitoring SDS residues at any time is of great significance.

The SDS Residue Detection Kit consists of three parts: 0.1% SDS solution, staining solution, and extraction solution. The 0.1% SDS solution can be diluted with water to prepare a gradient SDS standard solution and draw a standard curve. The staining solution can selectively bind with SDS in the sample to form a complex, which can be extracted into the organic phase by the extraction solution and then measured for OD value by UV spectrophotometry; The dye that has not been combined into a complex remains in the aqueous phase after extraction, so there is a certain linear relationship between the content of SDS in the sample and the absorption value of the complex extracted into the organic phase at a specific maximum absorption wavelength. It has been determined that the linear fit is good within the concentration range of 0.001% to 0.01% SDS, and can reach above 0.99.

 

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