Product Introduction:

Mucin Staining Kit (Mild Methylation Method) is a type of chemical modification and blocking method, which is based on the principle of eliminating the basophilicity of COOH by using an acidic alcohol solution.

Alcian staining solution at pH 2.5 ionizes the carboxyl groups within the tissue with a negative charge, forming a salt bond with the cations in Alcian blue, which stains tissues with carboxyl groups (e.g., proteoglycans/hyaluronic acid, and epithelial acidic mucins), and is primarily used to identify acidic groups in mucins. Untreated mucin sulfate, proteoglycan sulfate, salivary mucin, and hyaluronan stain blue, and treated sections stain light blue.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Experiment picture

Paraffin section of mouse small intestine
Untreated sulfated mucins, sulfated proteoglycans, sialomucins, and hyaluronic acid stain blue; after treatment, only sulfated mucins stain light blue.

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