Product Introduction:

Papanicolaou method is widely used in routine cytological staining. Papanicolaou stain was initially only used to detect estrogen level in vaginal epithelium and pathogens such as Candida and trichomonad in genital tract. The combination of orange G6 and EA36 or EA50 can dye the cytoplasm into bright green, blue and pink.

Sperm Morphology Stain Kit(Papanicolaou Method) can selectively combine with the corresponding dyes to dye sperm and proteins with different isoelectric points in the same acidity. The nucleus is composed of acidic substances, which have a strong affinity with alkaline dyes; on the contrary, the cytoplasm has a large affinity with alkaline substances and acid dyes. The cytoplasm is dyed with the modified EA50 staining solution specially for sperm staining, and the nucleus is dyed with the non-toxic modified hematoxylin staining solution independently developed.This kit is especially suitable for staining spermatozoa, and can also be used for staining cell samples such as pleural fluid, ascites and sputum.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Experiment picture

Smear of boar sperm
The acrosomal region of the sperm head appears light violet-blue; the post-acrosomal region appears violet-blue; the midpiece appears slightly red; the tail appears red or blue. Excess residual cytoplasm located below the head or around the midpiece stains orange-red (normal sperm have no residual cytoplasm, therefore no orange-red staining).

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