Assay Principle

This kit employs double antibody sandwich ELISA technology: capture antibody is coated on the microplate to capture VEGFR2 from samples and standards. After washing, biotin-labeled detection antibody is added and incubated, followed by washing to form a "capture antibody-antigen-detection antibody" immune-complex. Subsequently, Streptavidin-Horseradish Peroxidase (SA-HRP) is added and incubated. After incubation and washing, TMB substrate is added for color development. If the target analyte is present in the sample, a blue color develops. Stop solution is then added to terminate the reaction. During the assay, unbound components are washed away. The Optical Density (OD) is measured at 450 nm using a microplate reader. The intensity of the color is proportional to the VEGFR2 concentration in the sample, and the concentration is calculated by plotting a standard curve.

Typical Data

The following data and curve are for reference only. The experimenter must establish a standard curve based on their own experimental data.

 

Standard Conc. (pg/mL)	2000	1000	500	250	125	62.5	31.25	0
OD Value	2.707	1.533	0.795	0.423	0.226	0.132	0.087	0.037
Corrected OD Value	2.67	1.496	0.758	0.386	0.189	0.095	0.05	0

 

Sensitivity

The minimum detectable concentration (sensitivity) of Human VEGFR2, determined by testing samples, is 15.625pg/mL.

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