Product Description
       Annexins are a family of calcium-dependent phospholipid-binding proteins that preferentially bind phosphatidylserine (PS). Under normal physiologic conditions, PS is predominantly located in the inner leaflet of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution across the phospholipid bilayer and is translocated to the extracellular membrane leaflet marking cells as targets of phagocytosis. Once on the outer surface of the membrane, PS can be detected by fluorescently labeled Annexin V in a calcium-dependent manner.

    Note:This product is intended for adherent cells and compatible with most flow cytometers, except BD C6 and similar instruments with automatic voltage adjustment. For BD C6 and equivalent instruments, please use our C6-specific Annexin V-FITC/PI Apoptosis Kit.

 Product Data

Staining the Camptothecin-stimuLated HL-60 cells with Annexin V-FITC and PI, Total viable cells were used for analysis.

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