Core Detection Principle

The principle of combining liquid phase chip and double antibody sandwich is used. Two microspheres of different sizes (4μm and 5μm) with different fluorescence intensities are coated with antibodies specific to the detection index (capture microspheres), which can be specifically bound to the corresponding antigen proteins in the sample, and biotin-labeled detection antibodies are added to form an immune complex of antibody capture microspheres-cytokines-detection antibodies. Wash to remove excess unbound antibodies, add streptavidin-phycoerythrin conjugate (SA-PE), and detect the corresponding fluorescence on flow cytometry. The fluorescence intensity of the immune complex is used to obtain the content of the sample index to be tested.

 

Core advantages over traditional ELISA detection
 

Advantages:	Specific
Single tube multiple inspections	Simultaneous quantification of multiple target proteins (cytokines, chemokines, etc.) in one tube of sample, without the need to split the sample, and the data comparison is full
The sample loading volume is small	A single test only requires 25μL of samples, and small samples such as serum and plasma can also be accurately detected, suitable for rare samples
Efficient and fast	The whole process takes < 4 hours, and there is no need to stay up late for batch testing
High sensitivity	The lower limit of detection was as low as pg/mL, and high and low abundance proteins were captured simultaneously
Data stable	CV < 10%, avoiding false positives and meeting SCI publication requirements

Provide customized services (to meet individual needs)

 

Custom type	Detailed service content
Target customization	It supports single-target/multi-target combination customization to accurately match experimental needs
Large specification customization	Large specifications can be customized to adapt to large-scale sample experiments
Customized plan	Combine sample type (serum/plasma, etc.) and instrument model to customize exclusive experimental protocols to improve the success rate

 

Results shown 

 

 

                                        FSC&SSC                                                        Large particle size Beads                                         Small particle size beads

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