Sunlong Biotech™ Protein A(or Protein G) antibody purification beads are NHS activated superparamagnetic beads covalent binding Protein A (or Protein G).

 

Sunlong Biotech™ Protein A(or Protein G) antibody purification beads are NHS activated superparamagnetic beads covalent binding Protein A (or Protein G). Compared with the similar products in the international market, the product has higher antibody binding capacity and lower protein nonspecific adsorption rate, and the elution conditions are more homogeneous. The antibody can be separated from the serum sample by one-step purification, and the purity of the antibody is more than 90%. 

 

This product is micron magnetic beads, skilled operation can complete the antibody adsorption process within 15 min, and complete the process of antibody purification within 30 min. This product can be used repeatedly, which is suitable for the purification of antibodies in plasma, ascites, tissue culture supernatant and other samples. It can also be used for antibody fixation and other related studies. By referring the attached Table 1 users can select the categories of magnetic beads according to the source and subtype of the target antibody, and compare the affinity of Magrose Protein A and Magrose Protein G beads with different antibodies.

 

Product Information 

Item	Cat.No.60824	Cat.No.60824P	Cat.No.60814	Cat.No.60815
Product name	Magrose Protein A	Magrose Protein A	Magrose Protein A	Magrose Protein G
Bead size	10~30 μm	10~30 μm	30~150 μm	30~150 μm
Antibody binding ability (mg Human IgG/mL Gel)	40~45	45~50	25~30	25~30
Ligand	Protein A	Protein A	Protein A	Protein G
Beads Concentration	10% (V/V)
Storage buffer	PBS, 0.1% (V/V) Proclin 300, 0.1% (W/V) BSA		PBST, 0.1% (V/V) Proclin 300	PBST, 0.1% (V/V) Proclin 300
Medium	Magrose
Shelf life	2 years under 2~8℃ (able to be stored or transported for short under room temperature)
Binding/Washing buffer	PBST (pH 7.2-7.4): 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2.0 mM KH2PO4, 0.1% Tween-20	PBST (pH 7.2-7.4): 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2.0 mM KH2PO4, 0.1% Tween-20		PBST (pH 7.2-7.4): 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2.0 mM KH2PO4, 0.1% Tween-20
Elution buffer	100 mM Gly, 0.1% Tween-20, pH 2.0
Neutralization buffer	1.0 M Tris-HCl, pH 9.0

 

 

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