Drug Names

Generic Name: Rat ATPase copper transporting Beta (ATP7B)ELISA Kit

Purpose

Our Rat ATPase copper transporting Beta (ATP7B)ELISA kit is to assay ATP7B levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to ATP7B. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for ATP7B is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ATP7B and HRP conjugated ATP7B antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ATP7B. You can calculate the concentration of ATP7B in the samples by comparing the OD of the samples to the standard curve.

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