The Streptavidin Coated Beads, based on superparamagnetic or non-magnetic beads covalently coated with streptavidin, are designed to bind a variety of biotinylated molecules in immunoprecipitation, immunoassay(ELISA), cell sorting, protein interaction studies, protein/DNA hybridization reaction or even DNA sequencing application.

 

Sunlong Biotech^TM Streptavidin 280

 

1. Introduction

(SA-Biotin) system has extremely high binding affinity (Kd=10^-15), It has a wide range of applications in the biological field. Sunlong Biotech™ Streptavidin covalently connects the SA to the solid carrier surface by using the patented protein coupling technique of Sunlong, which can efficiently bind the ligands such as biotinylated antibodies, nucleic acids, proteins etc. The product adopts superparamagnetic microspheres with uniform size and regular morphology, which facilitates the rapid and convenient capture of target molecules and the realization of magnetic separation. This product can be equipped with automation equipment for high flux operation.

2. Basic Information

Index	Specification
Bead Size	2.8 μm
Color	Brown
Suspension time	>30 min
Biotinylated single stranded oligonucleotide (24nt )	600 pmol/mg beads
Biotinylated IgG	20 μg/mg beads

3. Figure

 

 

4. Acridine ester direct chemiluminescence

 

	Compare	Sunlong
S0	1045401	971434
S1	555277	538365
S2	360392	365044
S3	225030	227096
S4	135909	140109
S5	73068	81898
Clinic 1	374920	375358
Clinic 2	151969	160816

 

On FT4, Sunlong Biotech^TM shares the close measured value with that of the control magnetic beads, and the ratio is good。

5. ALP indirect chemiluminescence

Case 1 DAS-ELISA（HRP）MYO Test

MYO Standard ng/mL		SUNLONG	Competitor
250.0	64903596		50156262
125.0	41826343		33255404
62.5	22886909		18280646
31.2	11346377		8803140
15.6	5183601		3937511
7.8	2417043		1771504
3.9	1070908		770467
0	6947		7017

 

Sunlong Biotech^TM chemiluminescence performance is comparable to that of competitors’ magnetic products, which can be replaced, especially for low value samples, with better separation and more sensitive detection.

 

Case 2 DAS-ELISA（AP）tlgE Test

 

Conclusion：After calibration，the overall signal of Sunlong Biotech^TM is consistent with that of competing products, and linear regression R and slope are also ideal.

6.  Electrochemistry（PCT，DAS-ELISA）

PCT（DAS-ELISA）
Concentration（ng/mL）	Compare	Sunlong SA
0.026	956.7	1212
0.04	1038	1614
0.095	1379	3676
0.434	3841	15379
0.829	7048	32097
4.26	37719	183974
8.71	87416	326360
16.78	180826	666253
34.76	404879	1377481
62.78	775087	2375171
100	1313548	3969934

 

 

 

Conclusion：In PCT, Sunlong Biotech^TM has higher chemiluminescence signal, better separation and more sensitive detection.

7. Product List

Product Name	Size	Cat. No.
Sunlong BiotechTM COOH 280	2.8μm	70109
Sunlong BiotechTM Streptavidin 280	2.8μm	22309
Sunlong BiotechTM Streptavidin	300 nm	22308
Sunlong BiotechTM Streptavidin	1μm	22307

 

 

 

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