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Human Klebsiella Pneumoniae Toxin (KP-Toxin)ELISA Kit
Human Klebsiella Pneumoniae Toxin (KP-Toxin)ELISA Kit
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Regular members: $374.4
  • NO.:SL4764Hu
    Qualitative:Negative/Positive
    Sample Volume:10ul
    Wavelength:450nm
  • Goods click count:311
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Drug Names

Generic Name: Human Klebsiella Pneumoniae Toxin (KP-Toxin)ELISA Kit

Purpose

Our Human Klebsiella Pneumoniae Toxin (KP-Toxin)ELISA kit is for the qualitative determination of KP-Toxin in Human serum, plasma, culture media or any biological fluid.

Principle

The ELISA is based on the the qualitative enzyme immunoassay technique.The Microplate provided in this kit has been pre-coated with an Antibody specific to KP-Toxin, make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for KP-Toxin is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain KP-Toxin and HRP conjugated KP-Toxin antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of KP-Toxin is determined by comparing with the CUTOFF value.

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