Drug Names

Generic Name: Human L1 Cell Adhesion Molecule (L1CAM)ELISA Kit   

Purpose

Our Human L1 Cell Adhesion Molecule (L1CAM)ELISA Kit is to assay L1CAM levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to L1CAM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for L1CAM is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain L1CAM and HRP conjugated L1CAM antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of L1CAM. You can calculate the concentration of L1CAM in the samples by comparing the OD of the samples to the standard curve.

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