Summary:

 

Purpose

Our Quick Step Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT is to assay Col Ⅲ levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microplate provided in this kit has been pre-coated with an antibody specific to Col Ⅲ, make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Col Ⅲ is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain Col Ⅲ and HRP conjugated Col Ⅲ antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of Col Ⅲ is determined by comparing with the CUT OFF value.

Typical Data

The standard curve of QS0484Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.060	0.0560
		0.052
S1	7.2ng/ml	1.960	1.9575
		1.955
S2	4.8ng/ml	1.292	1.2875
		1.283
S3	2.4ng/ml	0.701	0.7045
		0.708
S4	1.2ng/ml	0.391	0.3920
		0.393
S5	0.6ng/ml	0.212	0.2045
		0.197

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