Summary:

 

Purpose

Our Quick Step human soluble cluster of differentiation 28 ,sCD28 ELISA Kit is to assay sCD28  levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microplate provided in this kit has been pre-coated with an antibody specific to sCD28 , make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for sCD28  is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain sCD28  and HRP conjugated sCD28  antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of sCD28  is determined by comparing with the CUT OFF value.

Typical Data

The standard curve of QS1596Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.060	0.0560
		0.052
S1	7.2ng/ml	1.960	1.9575
		1.955
S2	4.8ng/ml	1.292	1.2875
		1.283
S3	2.4ng/ml	0.701	0.7045
		0.708
S4	1.2ng/ml	0.391	0.3920
		0.393
S5	0.6ng/ml	0.212	0.2045
		0.197

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