Summary:

 

Purpose

Our Quick Step Rat NA-K-ATP ELISA Kit is to assay NA-K-ATP levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microplate provided in this kit has been pre-coated with an antibody specific to NA-K-ATP, make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for NA-K-ATP is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain NA-K-ATP and HRP conjugated NA-K-ATP antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of NA-K-ATP is determined by comparing with the CUT OFF value.

Typical Data

The standard curve of QS1222Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0.00U/ml	0.053	0.0525
		0.052
S1	6.00U/ml	1.951	1.9440
		1.937
S2	4.00U/ml	1.311	1.3150
		1.319
S3	2.00U/ml	0.718	0.7220
		0.726
S4	1.00U/ml	0.391	0.3890
		0.387
S5	0.50U/ml	0.213	0.2090
		0.205

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