Summary:

 

Purpose

Our Quick Step Human Tumor Marker DR-70 for lung cancer (DR-70TM)ELISA Kit is to assay DR-70TM levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microplate provided in this kit has been pre-coated with an antibody specific to DR-70TM, make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for DR-70TM is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain DR-70TM and HRP conjugated DR-70TM antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of DR-70TM is determined by comparing with the CUT OFF value.

Typical Data

The standard curve of QS1758Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0.00U/ml	0.053	0.0525
		0.052
S1	6.00U/ml	1.951	1.9440
		1.937
S2	4.00U/ml	1.311	1.3150
		1.319
S3	2.00U/ml	0.718	0.7220
		0.726
S4	1.00U/ml	0.391	0.3890
		0.387
S5	0.50U/ml	0.213	0.2090
		0.205

Cartpieces

• 
• 1

  1Delete

• 
• 8

  8Delete

• 
• Q

  QDelete

• 
• Q

  QDelete

• 
• +

  +Delete

• 
• 2

  2Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• Q

  QDelete

• Delete

• 
• <

  <Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• Q

  QDelete

• 
• 8

  8Delete

• 
• Q

  QDelete

Totalgoods,subtotals:￥Checkout