Summary:

 

Purpose

Our Quick Step Canine free tri-iodothyronine indes (FT3)ELISA Kit is to assay FT3 levels in Canine serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microplate provided in this kit has been pre-coated with an antibody specific to FT3, make it to solid-phase antibody.Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for FT3 is added to each Microplate well and incubated,so the antibody-antigen-Enzyme labeled antibody complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain FT3 and HRP conjugated FT3 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of FT3 is determined by comparing with the CUT OFF value.

Typical Data

The standard curve of QS0128Ca is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0.00pmol/L	0.053	0.0525
		0.052
S1	6.00pmol/L	1.951	1.9440
		1.937
S2	4.00pmol/L	1.311	1.3150
		1.319
S3	2.00pmol/L	0.718	0.7220
		0.726
S4	1.00pmol/L	0.391	0.3890
		0.387
S5	0.50pmol/L	0.213	0.2090
		0.205

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