Drug Names

Generic Name: Human S100 Calcium Binding Protein A7 (S100A7)ELISA Kit

Purpose

Our Human S100 Calcium Binding Protein A7 (S100A7)ELISA kit is to assay S100A7 levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to S100A7 Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for S100A7 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain S100A7 and HRP conjugated S100A7 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of S100A7 You can calculate the concentration of S100A7 in the samples by comparing the OD of the samples to the standard curve.

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