Product overview

Rat Complement fragment 3b (C3b) ELISA Kit (Catalog No. SL2014Ra) is an enzyme-linked immunosorbent assay kit for detection of the target in serum, plasma and cell culture supernatant.

Assay principle

The qualitative detection of Rat C3b antigen is based on the Double Antibody Sandwich ELISA (Enzyme-Linked Immunosorbent Assay) technique. The microplate wells are pre-coated with an antibody specific to Rat C3b. The corresponding antigen in the sample or standards binds the immobilized antibody after incubation. After washing the microplate wells to remove all unbound sample material, horseradish peroxidase (HRP) labeled antibody conjugate is added, which binds to the antigen, forming antibody-antigen enzyme-labeled antibody complex upon incubation. In a second wash step, all unbound material is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue color reaction in wells containing the complex. A stop solution containing acid terminates the reaction, producing a yellow end-point coloration proportional to the amount of target antigen present in the sample. Absorbance is measured at 450 nm using an ELISA microwell plate reader. Analysis is completed by comparing the OD values of samples to a cut-off

Specifications

• Assay range: 0.5ng/ml-40ng/ml
• Sensitivity: 0.1ng/ml
• Standard: 54ng/ml
• Sample volume: 10ul
• Wavelength: 450nm
• Size: 96T/48T

Kit components

• Sealed bags
• HRP-conjugate reagent
• Chromogen Solution A
• Chromogen Solution B
• Stop Solution

For research use only

Not for use in diagnostic or therapeutic procedures. Please refer to the instruction manual (PDF) for the complete protocol, sample preparation and calculation of results.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)