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Drug NamesGeneric Name: Rat Nuclear Receptor Coactivator 4 (NCOA4)ELISA Kit
Purpose
Our Rat Nuclear Receptor Coactivator 4 (NCOA4)ELISA kit is to assay NCOA4 levels in Rat serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to NCOA4. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for NCOA4 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain NCOA4 and HRP conjugated NCOA4 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of NCOA4. You can calculate the concentration of NCOA4 in the samples by comparing the OD of the samples to the standard curve.
+86 571 56623320
[email protected]
SUNLONG BIOTECH

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