Product overview

Human R Spondin 1 (RSPO1) ELISA Kit (Catalog No. SL4597Hu) is an enzyme-linked immunosorbent assay kit for detection of the target in serum, plasma and cell culture supernatant.

Specifications

• Assay range: 31.2pg/ml-2000pg/ml
• Sensitivity: 5.6pg/ml
• Standard: 2700pg/ml
• Sample volume: 10ul
• Wavelength: 450nm
• Size: 96T/48T

For research use only

Not for use in diagnostic or therapeutic procedures. Please refer to the instruction manual (PDF) for the complete protocol, sample preparation and calculation of results.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)

 Drug Names

Generic Name: Human R Spondin 1 (RSPO1)ELISA Kit

Purpose

Our Human R Spondin 1 (RSPO1)ELISA kit is to assay RSPO1 levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to RSPO1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for RSPO1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain RSPO1 and HRP conjugated RSPO1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of RSPO1. You can calculate the concentration of RSPO1 in the samples by comparing the OD of the samples to the standard curve.