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Simian Retro Virus IgG (SRV-IgG)ELISA Kit
Simian Retro Virus IgG (SRV-IgG)ELISA Kit
猿猴逆转录病毒IgG(SRV IgG)ELISA试剂盒
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Regular members: $422.4
  • NO.:SLY0063Mk
    Qualitative:Negative/Positive
    Method:Indirect ELISA
    Sample Volume:10ul
    Wavelength:450nm
  • Goods click count:567
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Details

 Purpose

Our Simian Retro Virus IgG (SRV-IgG)ELISA Kit is to for the qualitative determination of SRV-IgG in Simian serum, plasma, culture media or any biological fluid.

Principle

The ELISA is based on the the qualitative enzyme immunoassay technique.The Microplate provided in this kit has been pre-coated with an antigen specific to SRV-IgG, make it to solid-phase antigen.Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for SRV-IgG is added to each Microplate well and incubated,so the antigen-antibody-Enzyme labeled antigen complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain SRV-IgG and HRP conjugated SRV antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of SRV-IgG is determined by comparing with the CUT OFF value.

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