Aliases:	CDKN1B; Cyclin Dependent Kinase Inhibitor 1B; KIP1; P27KIP1; Cyclin-Dependent Kinase Inhibitor 1B (P27, Kip1); Cyclin-Dependent Kinase Inhibitor 1B; Cyclin-Dependent Kinase Inhibitor P27; P27Kip1; CDKN4; MEN1B; MEN4; P27
Background:	UniProt Entry: P46527;NCBI Gene Entry: 1027
Application Information	Molecular Weight: Predicted, 22 kDa, observed, 27 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1825 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human p27 KIP 1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Cyclin dependent kinase inhibitor 1B expression in HAP-1 cells using Cyclin dependent kinase inhibitor 1B antibody 1:2,000. Green, isotype control; red, Cyclin dependent kinase inhibitor 1B.

Immunocytochemical staining of HAP-1 cells with Cyclin dependent kinase inhibitor 1B antibody 1:1,000. Nuclei were stained blue with DAPI; Cyclin dependent kinase inhibitor 1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Cyclin dependent kinase inhibitor 1B antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:10,000 respectively.

Western blotting analysis using anti-Cyclin dependent kinase inhibitor 1B antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.