Aliases:	CRK; CRK Proto-Oncogene, Adaptor Protein; V-Crk Avian Sarcoma Virus CT10 Oncogene Homolog; Adapter Molecule Crk; Proto-Oncogene C-Crk; P38; V-Crk Sarcoma Virus CT10 Oncogene-Like Protein; CRKII
Background:	UniProt Entry: P46108;NCBI Gene Entry: 1398
Application Information	Molecular Weight: Predicted, 34 kDa, observed, 35 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1405 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CRKII
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of CRKII expression in HepG2 cells using CRKII antibody 1:2,000. Green, isotype control; red, CRKII.

Immunocytochemical staining of HepG2 cells with CRKII antibody 1:1,000. Nuclei were stained blue with DAPI; CRKII was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-CRKII antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-CRKII antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.